SEC-SAXS システム
◆装置概要
高圧溶液クロマトグラフィーを用いたゲルろ過カラムからの分画溶出液を直接小角X線散乱測定を行うことによって溶液中の大きさの異なるタンパク質、DNA、RNAの形状を効率的に測定できます。タンパク質単結晶X線解析、電子顕微鏡やMDシミュレーションと相補的に用いることで溶液中のタンパク質分子の詳細な動きを解析することができます。
◆装置の特徴
・小角X線散乱測定法によるタンパク質、DNA、RNAなどの構造解析が実施可能・高い統計精度と広い角度測定レンジでの小角X線散乱測定を迅速かつ簡便に測定
・異なるタンパク質が共存する溶液の各タンパク質の形状測定が可能
・試料ホルダーを変更することで、ポリマー材料などの連続自動測定も可能
◆実験・試料準備
被測定物の形状、サイズ
・20～500μLのタンパク質溶液、5 ㎜四方の生体由来試料、ポリマー材料、金属薄膜
SEC-SAXS System
◆Equipment overview
The shapes of proteins, DNA, and RNA of different sizes in the solution can be efficiently measured by directly performing small-angle X-ray scattering measurements of fractional elution from gel filtration columns using high-pressure solution chromatography. The detailed movement of protein molecules within a solution can be analysed by using it alongside single crystal protein X-ray analysis, an electron microscope, and MD simulation.
◆Features of the Equipment
・Structural analysis of proteins, DNA, RNA and more can be performed by small-angle X-ray scattering measurement methods.・It is possible to perform quick and easy measurements of small-angle X-ray scattering measurements with high-statistical accuracy and wide-angle measurements.
・It is possible to measure the shape of each protein within a solution in which different proteins coexist.
・Continuous automated measurements of polymer materials are possible by changing the sample holder.
◆Experiment / sample preparation
Shape and size of the objects to be measured:
・Protein solutions of 20～500μL, square biological samples of 5 mm, polymer materials and thin metal films.